|
Status |
Public on Oct 17, 2019 |
Title |
TH 661 |
Sample type |
RNA |
|
|
Source name |
Blood
|
Organism |
Homo sapiens |
Characteristics |
disease state: Univentricular Patient tissue: blood
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA including miRNAs was isolated using the Qiagen PAXgene™ Blood miRNA according to manufacturers recommendations.
|
Label |
using miRNA Complete Labeling and Hyb Kit (Agilent, Cat. No 5190-0456), Cyanine 3-pcp
|
Label protocol |
The expression profile of 2549 human miRNAs was determined using SurePrint G3 8x60k miRNA microarrays (Agilent) according to the manufacturers recommendations
|
|
|
Hybridization protocol |
The expression profile of 2549 human miRNAs was determined using SurePrint G3 8x60k miRNA microarrays (Agilent) according to the manufacturers recommendations
|
Scan protocol |
Array´s were scanned with the Agilent Scanner with default settings.
|
Description |
univentricular heart (UVH)
|
Data processing |
Raw expression values were extracted using the Feature Extraction Software of Agilent. Raw signal intensities were quantil-normalized
|
|
|
Submission date |
Aug 28, 2019 |
Last update date |
Oct 17, 2019 |
Contact name |
Masood Abu-Halima |
E-mail(s) |
masood@daad-alumni.de
|
Organization name |
Univeristy of Saarland
|
Department |
Human Genetics
|
Lab |
1.06
|
Street address |
Kirrberger St 60
|
City |
Homburg Saar |
ZIP/Postal code |
66421 |
Country |
Germany |
|
|
Platform ID |
GPL25134 |
Series (1) |
GSE136547 |
Micro-RNA 150-5p predicts overt heart failure in patients with univentricular hearts |
|