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Status |
Public on Apr 14, 2020 |
Title |
CDR=0.5 Plasma Sample 20 |
Sample type |
other |
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|
Source name |
CDR=0.5 Plasma Sample 20
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Organism |
Homo sapiens |
Characteristics |
individual: hip fracture repair (HFR) patient clinical dementia ranking (cdr): 0.5 age: 83.96167009 gender: MALE sample type: plasma
|
Extracted molecule |
other |
Extraction protocol |
All STRIDE hip fracture repair plasma samples were provided by Johns Hopkins University, and all control serum samples were purchased from BioServe, a commercial biofluid repositiory. All samples were drawn and stored under proper conditions, and collected and used with IRB approval.
|
Label |
Alexa Fluor 647
|
Label protocol |
Probing and analysis of microarrays was performed with strict adherence to suggested manufacturer protocols. (See Invitrogen and Molecular Devices online.)
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Hybridization protocol |
n/a
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Scan protocol |
Protein microarrays were scanned using a Genepix 4000B microarray scanner at 635nm using default parameters, PMT of 600, and a pixel size of 5um.
|
Description |
DEL CON-22
|
Data processing |
The GPR files were imported into Invitrogen's Prospector v5.2.3 microarray analysis software. Individual results were characterized into disease and control groups and then linearly normalized for group-to-group analysis of differential expression. Further significance analysis was performed using Random Forest (RF). [Matrix files] Normalized fluorescence intensities are presented in Relative Fluorescent Units (RFUs).
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Submission date |
Sep 13, 2019 |
Last update date |
Apr 14, 2020 |
Contact name |
Robert G Nagele |
E-mail(s) |
nagelero@rowan.edu
|
Phone |
856-566-6083
|
Organization name |
UMDNJ-SOM
|
Department |
NJISA
|
Lab |
Nagele
|
Street address |
2 Medical Center Drive
|
City |
Stratford |
State/province |
NJ |
ZIP/Postal code |
08084 |
Country |
USA |
|
|
Platform ID |
GPL27459 |
Series (1) |
GSE137422 |
Detection of early-stage Alzheimer’s pathology using blood-based autoantibody biomarkers in elderly hip fracture repair patients |
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