|
| Status |
Public on Apr 14, 2020 |
| Title |
Normal Control Donor Serum Sample 2 |
| Sample type |
other |
| |
|
| Source name |
Normal Control Donor Serum Sample 2
|
| Organism |
Homo sapiens |
| Characteristics |
individual: control
age: 59
gender: FEMALE
sample type: serum
|
| Extracted molecule |
other |
| Extraction protocol |
All STRIDE hip fracture repair plasma samples were provided by Johns Hopkins University, and all control serum samples were purchased from BioServe, a commercial biofluid repositiory. All samples were drawn and stored under proper conditions, and collected and used with IRB approval.
|
| Label |
Alexa Fluor 647
|
| Label protocol |
Probing and analysis of microarrays was performed with strict adherence to suggested manufacturer protocols. (See Invitrogen and Molecular Devices online.)
|
| |
|
| Hybridization protocol |
n/a
|
| Scan protocol |
Protein microarrays were scanned using a Genepix 4000B microarray scanner at 635nm using default parameters, PMT of 600, and a pixel size of 5um.
|
| Description |
BioS-CT30
|
| Data processing |
The GPR files were imported into Invitrogen's Prospector v5.2.3 microarray analysis software. Individual results were characterized into disease and control groups and then linearly normalized for group-to-group analysis of differential expression. Further significance analysis was performed using Random Forest (RF).
[Matrix files] Normalized fluorescence intensities are presented in Relative Fluorescent Units (RFUs).
|
| |
|
| Submission date |
Sep 13, 2019 |
| Last update date |
Apr 14, 2020 |
| Contact name |
Robert G Nagele |
| E-mail(s) |
nagelero@rowan.edu
|
| Phone |
856-566-6083
|
| Organization name |
UMDNJ-SOM
|
| Department |
NJISA
|
| Lab |
Nagele
|
| Street address |
2 Medical Center Drive
|
| City |
Stratford |
| State/province |
NJ |
| ZIP/Postal code |
08084 |
| Country |
USA |
| |
|
| Platform ID |
GPL27459 |
| Series (1) |
| GSE137422 |
Detection of early-stage Alzheimer’s pathology using blood-based autoantibody biomarkers in elderly hip fracture repair patients |
|
