|
| Status |
Public on Jun 05, 2020 |
| Title |
AN10: patient 25 |
| Sample type |
RNA |
| |
|
| Source name |
conjunctival cells
|
| Organism |
Homo sapiens |
| Characteristics |
age: 20
gender: F
disease: AAK
Stage: Severe
tissue: conjunctival cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA including miRNAs was isolated using the Qiagen miRNeasy Kit according to manufacturers recommendations.
|
| Label |
Cy3
|
| Label protocol |
miRNA Complete Labeling and Hyb Kit (Agilent, Cat. No 5190-0456)
|
| |
|
| Hybridization protocol |
The expression profile of 2549 human miRNAs was determined using SurePrint G3 8x60k miRNA microarrays (Agilent) according to the manufacturers recommendations
|
| Scan protocol |
Array´s were scanned with the Agilent Scanner with default settings.
|
| Description |
miRNAs in embryonic culture medium
|
| Data processing |
Raw expression values were extracted using the Feature Extraction Software of Agilent. Raw signal intensities were quantil-normalized and log2 transformed.
|
| |
|
| Submission date |
Sep 25, 2019 |
| Last update date |
Jun 05, 2020 |
| Contact name |
Nicole Ludwig |
| E-mail(s) |
n.ludwig@mx.uni-saarland.de
|
| Phone |
004968411626269
|
| Organization name |
Saarland University
|
| Department |
Human Genetics
|
| Street address |
Kirrberger Strasse
|
| City |
Homburg |
| ZIP/Postal code |
66421 |
| Country |
Germany |
| |
|
| Platform ID |
GPL25134 |
| Series (2) |
| GSE137995 |
Conjunctival mRNA and miRNA expression profiles in congenital aniridia are genotype and phenotype dependent (AKK miRNA) |
| GSE137997 |
Conjunctival mRNA and miRNA expression profiles in congenital aniridia are genotype and phenotype dependent |
|
