|
Status |
Public on Jun 05, 2020 |
Title |
AN14: patient 26 |
Sample type |
RNA |
|
|
Source name |
conjunctival cells
|
Organism |
Homo sapiens |
Characteristics |
age: 28 gender: F disease: AAK Stage: Mild tissue: conjunctival cells
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA including miRNAs was isolated using the Qiagen miRNeasy Kit according to manufacturers recommendations.
|
Label |
Cy3
|
Label protocol |
miRNA Complete Labeling and Hyb Kit (Agilent, Cat. No 5190-0456)
|
|
|
Hybridization protocol |
The expression profile of 2549 human miRNAs was determined using SurePrint G3 8x60k miRNA microarrays (Agilent) according to the manufacturers recommendations
|
Scan protocol |
Array´s were scanned with the Agilent Scanner with default settings.
|
Description |
miRNAs in embryonic culture medium
|
Data processing |
Raw expression values were extracted using the Feature Extraction Software of Agilent. Raw signal intensities were quantil-normalized and log2 transformed.
|
|
|
Submission date |
Sep 25, 2019 |
Last update date |
Jun 05, 2020 |
Contact name |
Nicole Ludwig |
E-mail(s) |
n.ludwig@mx.uni-saarland.de
|
Phone |
004968411626269
|
Organization name |
Saarland University
|
Department |
Human Genetics
|
Street address |
Kirrberger Strasse
|
City |
Homburg |
ZIP/Postal code |
66421 |
Country |
Germany |
|
|
Platform ID |
GPL25134 |
Series (2) |
GSE137995 |
Conjunctival mRNA and miRNA expression profiles in congenital aniridia are genotype and phenotype dependent (AKK miRNA) |
GSE137997 |
Conjunctival mRNA and miRNA expression profiles in congenital aniridia are genotype and phenotype dependent |
|