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Sample GSM4107989 Query DataSets for GSM4107989
Status Public on Mar 09, 2021
Title Acute myeloid leukemia cell line KG-1
Sample type genomic
 
Source name Acute myeloid leukemia cell line KG-1
Organism Homo sapiens
Characteristics cell line: KG-1
cell type: Acute myeloid leukemia cell line
Treatment protocol All cell lines were untreated prior to SNP analysis.
Growth protocol KG-1 and U937 were grown in RPMI supplemented with 10 % fetal calf serum and 1 % penicillin/streptomycin. UCSD-AML1 and ELF-153 were grown in RPMI supplemented with 20 % fetal calf serum, 1 % penicillin/streptomycin and 5 ng/ml human GM-CSF.
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted using the Quiagen DNeasy Blood and Tissue DNA extraction Kit according to manufacturer's protocol.
Label biotin
Label protocol Samples were labeled using the Affymetrix CytoScan Reagent and the SNP 6.0 Core Reagent Kits (USCD-AML1/ELF-153/KG-1 and U937, respectively)
 
Hybridization protocol according to manufacturer (Affymetrix)
Scan protocol according to manufacturer (Affymetrix)
Data processing CEL files were analysed using the Affymetrix Chromosome Analysis Suite ChAS (USCD-AMl1/ELF-153/KG-1) and the Affymetrix Genoyping Console Software (U937); data was annotated to the reference genome assembly GRCh37(hg19).
 
Submission date Oct 04, 2019
Last update date Mar 09, 2021
Contact name Gabriele Greve
E-mail(s) gabriele.greve@uniklinik-freiburg.de
Organization name University of Freiburg Medical Center
Department Department of Internal Medicine 1
Lab Division of Hematology, Oncology and SCT, Lab Prof. Michael Lübbert
Street address Hugstetter Str 55
City Freiburg
ZIP/Postal code 79106
Country Germany
 
Platform ID GPL18637
Series (1)
GSE138438 Copy-number profiling by SNP array of 4 acute myeloid leukemia (AML) cell lines

Supplementary file Size Download File type/resource
GSM4107989_KG1.CEL.gz 20.5 Mb (ftp)(http) CEL
GSM4107989_KG1.cy750K.cychp.gz 21.9 Mb (ftp)(http) CYCHP
Processed data provided as supplementary file

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