|
| Status |
Public on Mar 30, 2020 |
| Title |
257015613554_1_2 [S-2-2] |
| Sample type |
RNA |
| |
|
| Source name |
serum of radiosensitive NPC patient
|
| Organism |
Homo sapiens |
| Characteristics |
subject status: Nasopharyngeal carcinoma (NPC) patient
subject group: radiosensitive
age: 49y
gender: male
tumor stage: II
tissue: serum
|
| Growth protocol |
About 5mL of non-coagulant blood was collected from every eligible patient. Serum was isolated by centrifuging at 3000 rpm for 10 min at 4 °C, and stored at −80 °C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was prepared using the TRIzol reagent following the manufacturer's recommendations. RNA was quantified using a NanoDrop-1000 spectrophotometer and quality was monitored with the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA).
|
| Label |
Cy3
|
| Label protocol |
Labeled RNAs were heat denatured and hybridized to Agilent miRNA microarray, according to manufacturer's protocol.
|
| |
|
| Hybridization protocol |
According to the Agilent miRNA Microarray Protocol (Agilent Technologies).
|
| Scan protocol |
After hybridization and post-hybridization washes, slides were scanned immediately in Agilent Microarray Scanner with Surescan High Resolution Technology (Agilent Technologies, Santa Clara, CA).
|
| Description |
Serum miRNA expression of NPC
|
| Data processing |
Feature Extraction v10.7.3.1 (Agilent Technologies, CA) software was used to extract all features of the data obtained from the scanned images and Bioconductor software was used to analyze the raw data, which are normalized by quantile normalization.
|
| |
|
| Submission date |
Oct 21, 2019 |
| Last update date |
Mar 30, 2020 |
| Contact name |
Kaiguo Li |
| Organization name |
Guangxi Medical University Cancer Hospital
|
| Department |
Department of Radiation Oncology
|
| Street address |
71 Hedi Road
|
| City |
Nanning |
| State/province |
Guangxi Zhuang Autonomous Region |
| ZIP/Postal code |
530021 |
| Country |
China |
| |
|
| Platform ID |
GPL25134 |
| Series (1) |
| GSE139164 |
Serum miRNA expression profile of nasopharyngeal carcinoma with different radiosensitivity |
|
