|
| Status |
Public on Jun 09, 2009 |
| Title |
lung_AIRmin_male_urethane_mice15 |
| Sample type |
RNA |
| |
|
| Source name |
lung from AIRmin male treated with urethane
|
| Organism |
Mus musculus |
| Characteristics |
tissue: lung
strain: AIRmin
gender: M
agent: urethane
|
| Treatment protocol |
two intraperitoneal injections of 1 mg/g body weight urethane or saline solution
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with RNeasy Midi kit (Qiagen, Valencia, CA) from normal lung parenchyma and purified with RNeasy MinElute CleanUP Kit (Qiagen). RNA concentration was determined with a Nanodrop spectrophotometer (NanoDrop, Wilmington, DE) and RNA quality was assessed with an Agilent 2100 Bioanalyzer (Agilent Technologies Inc., Santa Clara, CA)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
no additional information
|
| Data processing |
The data were normalised using cubic spline normalization with Illumina's BeadStudio Gene Expression Module v3.4, July 2008 (see VALUE column below).
The data were then filtered by detection p-value (p<0.05). A matrix of the normalized, filtered data is linked as supplementary file at the foot of the Series record.
|
| |
|
| Submission date |
Jun 09, 2009 |
| Last update date |
Jun 09, 2009 |
| Contact name |
Loris De Cecco |
| E-mail(s) |
loris.dececco@istitutotumori.mi.it
|
| Organization name |
IRCSS Istituto Nazionale Tumori
|
| Street address |
via Venezian 1
|
| City |
Milan |
| ZIP/Postal code |
20133 |
| Country |
Italy |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE16510 |
Normal lung transcriptome distinguishes mouse lines with different susceptibility to inflammation and to tumorigenesis |
|
