|
| Status |
Public on Jun 10, 2009 |
| Title |
TIL #22 CD8+CD28- |
| Sample type |
RNA |
| |
|
| Source name |
CD8+CD28-
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: human melanoma tumor infiltrating lymphocytes
|
| Treatment protocol |
FACS sorting
|
| Growth protocol |
TIL was isolated out of melanoma tumor fragment during 5 weeks culture in presence of IL-2; then subjected to rapid expansion protocol where CD3 stimulation and high dose IL-2 provided; TIL were harvested and sorted based on their CD8+ and CD28 expression. Isolation (5 weeks) -> expansion (2 weeks) -> sorting.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
RNA from sorted TIL sample
|
| Data processing |
The data were normalised using BeadStudio software from Illumina
|
| |
|
| Submission date |
Jun 09, 2009 |
| Last update date |
Jun 09, 2009 |
| Contact name |
yufeng li |
| E-mail(s) |
yufenli@mdanderson.org
|
| Phone |
7135630696
|
| Organization name |
MDACC
|
| Department |
Melanoma
|
| Street address |
7455 Fannin
|
| City |
houston |
| State/province |
TX |
| ZIP/Postal code |
77054 |
| Country |
USA |
| |
|
| Platform ID |
GPL6884 |
| Series (1) |
| GSE16517 |
Human CD28+/- tumor infiltrating lymphocytes gene expression |
|
