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Sample GSM416733

Query DataSets for GSM416733

Status

Public on Aug 25, 2009

Title

HEK293

Sample type

SRA

Source name

cell line

Organism

Homo sapiens

Characteristics

cell line: HEK293

Growth protocol

RNA was harvested from exponentially growing cells

Extracted molecule

total RNA

Extraction protocol

Total RNA extracted using standard Trizol extraction. Total RNA fractionated on 15% polyacrylamide gel and 3' and 5' adaptors sequentially ligated requiring a 3' hydroxyl group and 5' phosphate

Library strategy

RNA-Seq

Library source

transcriptomic

Library selection

size fractionation

Instrument model

Illumina Genome Analyzer

Description

none

Data processing

Reads containing linker matches were mapped (after removing linkers) to predicted pre-miRNAs (MirBase version 12.0)

Submission date

Jun 12, 2009

Last update date

Jun 11, 2013

Contact name

Christine Mayr

E-mail(s)

mayrc@mskcc.org

Organization name

Whitehead Institute

Street address

9 Cambridge Center

City

Cambridge

State/province

ZIP/Postal code

02142

Country

USA

Platform ID

GPL9052

Series (1)

GSE16579

miRNA expression of human cancer and mouse cell lines

Relations

BioSample

SAMN02197711

Data table header descriptions
SEQUENCE
COUNT	counts

Data table
SEQUENCE	COUNT
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	31756
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC	88
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG	20
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT	9
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACA	166
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC	2
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACT	1
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGA	18
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAANA	542
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAANN	1
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA	7
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAA	183
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAC	7
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAG	1
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAT	4
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCA	8
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACNA	1
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAA	20
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAG	3
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGCA	2

Total number of rows: 541755

Table truncated, full table size 20651 Kbytes.

Supplementary data files not provided

Processed data included within Sample table