|
| Status |
Public on Dec 11, 2020 |
| Title |
P/MS [881SP182] |
| Sample type |
RNA |
| |
|
| Source name |
PBMC from P/MS
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Male
age (years): 31.00
|
| Treatment protocol |
PBMC were purified from Ficoll hypaque gradient (Invitrogen, USA)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
PBMC gene expression from P/MS patients
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
The Supplementary Tables 1-3 included statistically significant genes obtained by comparison of each experimental group Multiple Sclerosis only (MSO), Psoriasis only (PSO), Psoriasis and Multiple Sclerosis comorbid patients (P/MS) with Healthy Controls (HC) The Supplementary Table 4 include upstream regulators for PSO related genes.
Supplementary Table 1 - "MSO Vs HC"
Supplementary Table 2 - "PSO Vs HC"
Supplementary Table 3 - "P/MS Vs HC"
Supplementary Table 4 - "Upstream regulators for PSO genes"
|
| |
|
| Submission date |
Dec 11, 2019 |
| Last update date |
Dec 12, 2020 |
| Contact name |
Michael Gurevich |
| E-mail(s) |
Michael.Gurevich@sheba.health.gov.il
|
| Organization name |
Sheba Medical Center
|
| Department |
Multiple Sclerosis Center
|
| Lab |
Neurogenomics and Neuroimmunology
|
| Street address |
Tel Hashomer
|
| City |
Ramat Gan |
| ZIP/Postal code |
52621 |
| Country |
Israel |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE141804 |
Co-Morbid Multiple Sclerosis and Psoriasis: Clinical Outcomes and Gene Expression |
|
