|
| Status |
Public on Mar 31, 2010 |
| Title |
EP4KO (KO or Tg) #11 - high EF |
| Sample type |
RNA |
| |
|
| Source name |
left ventricle
|
| Organism |
Mus musculus |
| Characteristics |
strain: mixed genetic background
age (weeks): 33.5
ef%: 60.2
sex: male
tissue: left ventricle
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using an RNAeasy fibrous tissue mini kit (Qiagen), followed by clean-up and DNase I treatment in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent 2100 Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNAs were prepared with the Illumina TotalPrep RNA amplification kit.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol with BeadStation 500 GX with scan settings at the default settings for Direct Hybridization.
|
| Description |
058-B
|
| Data processing |
Raw expression data of the 24 mice from the Illumina BeadStudio software were processed using the lumi program (version 1.6.3), which is an implemented package designed for processing Illumina microarrays in R (version 2.8.0) [http://www.R-project.org.], the open source environment of statistical analysis. The raw intensities from each mouse sample were transformed using variance stabilization transformation (VST), which takes advantage of the multiple replicates available on the Illumina arrays.
|
| |
|
| Submission date |
Jun 30, 2009 |
| Last update date |
Mar 31, 2010 |
| Contact name |
Margot C LaPointe |
| E-mail(s) |
mlapoin1@hfhs.org
|
| Phone |
313-916-3076
|
| Fax |
313-916-1479
|
| Organization name |
Henry Ford Hospital
|
| Department |
Internal Medicine
|
| Lab |
Hyp. and Vasc. Res. Div.
|
| Street address |
2799 W. Grand Blvd.
|
| City |
Detroit |
| State/province |
MI |
| ZIP/Postal code |
48202-2689 |
| Country |
USA |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE16909 |
Gene expression profiling of a mouse model of dilated cardiomyopathy |
|
