|
| Status |
Public on Sep 23, 2009 |
| Title |
Dicer KO replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
Embryonic Stem Cells
|
| Organism |
Mus musculus |
| Characteristics |
cell type: embryonic stem cells
genetic modification: Dicer knockout
|
| Growth protocol |
Wild-type, dgcr8 knockout, and dicer1 knockout ES cells were plated on 60-mm plates in triplicate.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Gladstone Genomic Core facility standard protocols were used (http://www.gladstone.ucsf.edu/gladstone/site/genomicscore/section/1914).
|
| |
|
| Hybridization protocol |
Gladstone Genomic Core facility standard protocols were used (http://www.gladstone.ucsf.edu/gladstone/site/genomicscore/section/1914).
|
| Scan protocol |
Gladstone Genomic Core facility standard protocols were used (http://www.gladstone.ucsf.edu/gladstone/site/genomicscore/section/1914).
|
| Description |
Gene expression data from Dicer knockout ES cells
|
| Data processing |
Data were normalized using the GCRMA algorithm.
|
| |
|
| Submission date |
Jul 01, 2009 |
| Last update date |
Sep 23, 2009 |
| Contact name |
Robert Blelloch |
| E-mail(s) |
blellochr@stemcell.ucsf.edu
|
| Phone |
415-476-2838
|
| Organization name |
University of California, San Francisco
|
| Street address |
513 Parnassus Avenue, Campus Box #0525
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94143 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE16923 |
Expression data from wild-type, Dgcr8 knockout and Dicer knockout ES cells |
|
