|
| Status |
Public on Apr 19, 2010 |
| Title |
Stage II colorectal cancer tissue samples (FFPE) 33 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
colorectal cancer
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: colorectal cancer
tumor stage: stage II
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA isolation protocol available via www.vumc.nl/microarrays. Also described in Buffart et al, J Pathol. 2007 Jan;211(1):45-51
|
| Label |
Cy3
|
| Label protocol |
DNA labeling protocol according to Enzo Life Sciences
|
| |
|
| Channel 2 |
| Source name |
normal colorectum mucosa
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: normal colon mucosa obtained from the same patient as Channel 1
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA isolation protocol available via www.vumc.nl/microarrays. Also described in Buffart et al, J Pathol. 2007 Jan;211(1):45-51
|
| Label |
Cy5
|
| Label protocol |
DNA labeling protocol according to Enzo Life Sciences
|
| |
|
| |
| Hybridization protocol |
Hybridization protocol available via www.agilent.com
|
| Scan protocol |
Slides were scanned using the Agilent microarray scanner, protocol according to Agilent.
|
| Description |
Normal colon mucosa from each patient was used as reference in the oaCGH experiments
|
| Data processing |
Median Signal intensity minus median Background intensity; excluding negative values
|
| |
|
| Submission date |
Jul 10, 2009 |
| Last update date |
Apr 19, 2010 |
| Contact name |
Daoud Sie |
| E-mail(s) |
d.sie@vumc.nl
|
| Phone |
+31 20 4442428
|
| Organization name |
Vrije Universiteit Medical Center
|
| Department |
Pathology
|
| Lab |
Microarray Core Facility
|
| Street address |
De Boelelaan 1117
|
| City |
Amsterdam |
| ZIP/Postal code |
1081 HV |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL8841 |
| Series (2) |
| GSE17047 |
Candidate driver genes in focal chromosomal aberrations of stage II colon cancer |
| GSE17181 |
Deletion of chromosome 4q predicts outcome in Stage II colon cancer |
|
