|
| Status |
Public on Dec 01, 2009 |
| Title |
B10M75 |
| Sample type |
RNA |
| |
|
| Source name |
Leukocytes, Boutroch, Rural
|
| Organism |
Homo sapiens |
| Characteristics |
geographic location: Boutroch
lifestyle: rural
gender: male
tissue: peripheral blood
cell type: leukocytes
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extrated from leukocyte samples using Ambion's Leukolocks kit. Quality control was performed using Agilent's Bioanalyzer.
|
| Label |
Biotin
|
| Label protocol |
cDNA and cRNA labeling and amplification were all performed using a single kit: Ambion's Illumina TotalPrep RNA Amplification kit.
|
| |
|
| Hybridization protocol |
Illumina's BeadArray protocol
|
| Scan protocol |
Illumina's BeadArray protocol and BeadStudio (Gene expression Module)
|
| Description |
B10M
|
| Data processing |
Raw data was log2 transformed and median-centered using JMP Genomics (SAS, Cary NC).
|
| |
|
| Submission date |
Jul 13, 2009 |
| Last update date |
Nov 12, 2009 |
| Contact name |
Youssef Idaghdour |
| E-mail(s) |
yidaghd@ncsu.edu
|
| Organization name |
NCSU
|
| Department |
Genetics
|
| Lab |
Gibson
|
| Street address |
South Gardner Hall
|
| City |
Raleigh |
| State/province |
NC |
| ZIP/Postal code |
27695 |
| Country |
USA |
| |
|
| Platform ID |
GPL6947 |
| Series (1) |
| GSE17065 |
Geographical Genomics of Human Leukocytes Gene Expression Variation |
|
