|
| Status |
Public on Jul 05, 2010 |
| Title |
LPS treated BV-2 cells |
| Sample type |
RNA |
| |
|
| Source name |
mouse brain microglia
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
|
| Treatment protocol |
BV-2 cells were treated with LPS in the absence or presence of obovatol for 6 hr.
|
| Growth protocol |
The immortalized BV-2 murine microglia cell line was maintained in Dulbecco’s modified Eagle’s medium (DMEM) containing 5% heat-inactivated FBS, 50 μg/ml gentamicin at 37°C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
LPS-treated sample
|
| Data processing |
Images were quantified using software program Illumina beadscan 3.2 (Illumina Inc., San Diego, CA) and BeadStudio v3.0 (Illumina Inc.).
|
| |
|
| Submission date |
Jul 16, 2009 |
| Last update date |
Jul 05, 2010 |
| Contact name |
Kyoungho Suk |
| E-mail(s) |
ksuk@knu.ac.kr
|
| Organization name |
Kyungpook National University School of Medicine
|
| Department |
Department of Pharmacology
|
| Street address |
101 Dong-In, Joong-gu
|
| City |
Daegu |
| ZIP/Postal code |
700-422 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE17069 |
Genome-wide analysis of effects of obovatol on lipopolysaccharide (LPS)-induced gene expression in microglia |
|
