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Sample GSM434616 Query DataSets for GSM434616
Status Public on Feb 05, 2010
Title FT.3-rep.2
Sample type RNA
 
Source name EpiSC from the epiblast of a fertilized embryo
Organism Mus musculus
Characteristics strain: B6D2
embryo stage: pre-streak/early streak
embryo morphology: normal
Treatment protocol none
Growth protocol EpiSC were maintained on FBS coated dishes in Completely Defined Medium complemented with 20 ng/mL activine A (R&D) and 12 ng/mL bFgF (R&D) according to Brons et al., 2007.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted with the QIAGEN RNeasy minikit followed by DNAse I treatment in accordance with the manufacturer instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description n/a
Data processing After exported from BeadStudio, data were normalised using lumiExpresso provided in lumi R package
 
Submission date Jul 29, 2009
Last update date Dec 23, 2009
Contact name Julien MARUOTTI
E-mail(s) maruotti@inapg.fr
Organization name INRA
Lab BDR-Bat 230
Street address Domaine de Vilvert
City JOUY EN JOSAS
ZIP/Postal code 78352
Country France
 
Platform ID GPL6887
Series (1)
GSE17402 Genome-wide comparison of EpiSC lines derived from fertilized (FT) and somatic cell nuclear transfer (NT) embryos

Data table header descriptions
ID_REF
VALUE Background correction + Variance Stabilization (vst) + Quantile normalization

Data table
ID_REF VALUE
ILMN_2735294 8.453275815
ILMN_2417611 7.762638826
ILMN_2545897 8.010775746
ILMN_2762289 7.819003244
ILMN_1248788 7.968288973
ILMN_2707227 8.044510802
ILMN_2896528 12.60738181
ILMN_2721178 10.83709724
ILMN_1227723 9.327448145
ILMN_2458837 7.790931881
ILMN_3033922 10.06096621
ILMN_3092673 11.81782818
ILMN_1230777 10.9835059
ILMN_2730714 12.28896648
ILMN_1246069 12.62443131
ILMN_1232042 7.763522697
ILMN_1243193 10.63894524
ILMN_2524361 7.957671696
ILMN_1233188 8.20423086
ILMN_2543688 8.686337468

Total number of rows: 45281

Table truncated, full table size 1100 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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