|
| Status |
Public on Oct 29, 2020 |
| Title |
human embryonic stem cell line derived after Cas9 RPN injection at fertilization_eysES4_rep 4 |
| Sample type |
genomic |
| |
|
| Source name |
pluripotent stem cell line eysES4, gamete combination egg donor C and sperm donor
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: pluripotent stem cell line
gender: female
|
| Treatment protocol |
Cas9 guide RNA ribonucleoprotein complex was assembled in vitro and injected either with sperm at MII stage, or at the 2-cell stage on day2 after ICSI
|
| Growth protocol |
fertilization of human metaphaseII oocytes was performed using standard intracytoplasmic sperm injection (ICSI), followed by culture in GlobalTotal (LifeGlobal)
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
single cells were placed into single PCR tubes containing 2microliter of PBS or other hypotonic collection buffer. Genomic DNA amplification was performed using amplification kit as indicated for each sample.
|
| Label |
biotin
|
| Label protocol |
Affymetrix UK Biobank Axiom Array Manufacturer's Protocol
|
| |
|
| Hybridization protocol |
Affymetrix UK Biobank Axiom Array Manufacturer's Protocol
|
| Scan protocol |
Affymetrix UK Biobank Axiom Array Manufacturer's Protocol
|
| Description |
eysES4 rep 4
|
| Data processing |
Affymetrix UK Biobank Axiom Array Manufacturer's Protocol
processed data files include DNA copy number and genotyping
|
| |
|
| Submission date |
Apr 10, 2020 |
| Last update date |
Oct 30, 2020 |
| Contact name |
Nathan Treff |
| Organization name |
Genomic Prediction, Inc.
|
| Street address |
675 US Highway One
|
| City |
North Brunswick Township |
| State/province |
NJ |
| ZIP/Postal code |
08902 |
| Country |
USA |
| |
|
| Platform ID |
GPL28377 |
| Series (1) |
| GSE148488 |
SNP array for Cas9 treated human oocytes and embryos |
|
