|
Status |
Public on Oct 29, 2020 |
Title |
Cleavage stage blastomere_day4_Cas9 RNP injection at fertilization_embryo A_biopsy 3_ rep1 |
Sample type |
genomic |
|
|
Source name |
blastomere from gamete combination egg donor D and sperm donor
|
Organism |
Homo sapiens |
Characteristics |
gender: nd
|
Treatment protocol |
Cas9 guide RNA ribonucleoprotein complex was assembled in vitro and injected either with sperm at MII stage, or at the 2-cell stage on day2 after ICSI
|
Growth protocol |
fertilization of human metaphaseII oocytes was performed using standard intracytoplasmic sperm injection (ICSI), followed by culture in GlobalTotal (LifeGlobal)
|
Extracted molecule |
genomic DNA |
Extraction protocol |
single cells were placed into single PCR tubes containing 2microliter of PBS or other hypotonic collection buffer. Genomic DNA amplification was performed using amplification kit as indicated for each sample.
|
Label |
biotin
|
Label protocol |
Affymetrix UK Biobank Axiom Array Manufacturer's Protocol
|
|
|
Hybridization protocol |
Affymetrix UK Biobank Axiom Array Manufacturer's Protocol
|
Scan protocol |
Affymetrix UK Biobank Axiom Array Manufacturer's Protocol
|
Description |
kit used for genomic DNA amplification: REPLI-g single cell kit (Qiagen Cat #150345)
|
Data processing |
Affymetrix UK Biobank Axiom Array Manufacturer's Protocol processed data files include DNA copy number and genotyping
|
|
|
Submission date |
May 14, 2020 |
Last update date |
Oct 30, 2020 |
Contact name |
Nathan Treff |
Organization name |
Genomic Prediction, Inc.
|
Street address |
675 US Highway One
|
City |
North Brunswick Township |
State/province |
NJ |
ZIP/Postal code |
08902 |
Country |
USA |
|
|
Platform ID |
GPL28377 |
Series (1) |
GSE148488 |
SNP array for Cas9 treated human oocytes and embryos |
|