|
| Status |
Public on May 15, 2010 |
| Title |
P130 UK normal prostate |
| Sample type |
genomic |
| |
|
| Source name |
UK normal prostate
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Normal prostate
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Fresh frozen samples were digested by Proteinase K and RNaseA. DNA was extracted from these samples using phenol/chroloform.
|
| Label |
Biotin
|
| Label protocol |
As per manufacturer (Affymetrix)
|
| |
|
| Hybridization protocol |
DNA was restriction digested, PCR amplified, fragmented, labeled and hybridized to each array according to the manufacturer's instructions.
|
| Scan protocol |
The Arrays were then washed using Affymetrix fluidics stations, and scanned using the Gene Chip Scanner 3000 7G.
|
| Description |
Hybridized to human SNP 6.0 array
|
| Data processing |
CEL files and CHP files generated by Affymetrix Genechip Operating Software. Very weakly hybridized SNPs were filtered by the same software. The signal intensity data from SNP array were analyzed by in-house software- GOLF and commerical software-Partek.
|
| |
|
| Submission date |
Sep 29, 2009 |
| Last update date |
Mar 05, 2010 |
| Contact name |
Xueying Mao |
| E-mail(s) |
x.mao@qmul.ac.uk
|
| Organization name |
Queen Mary
|
| Street address |
Charterhouse Square
|
| City |
London |
| ZIP/Postal code |
EC1M 6BQ |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL6801 |
| Series (1) |
| GSE18333 |
Distinct genomic differences in prostate cancer between Western countries and China |
|
