|
| Status |
Public on Oct 20, 2009 |
| Title |
Vehicle |
| Sample type |
SAGE |
| Anchor |
NlaIII
|
| Tag Count |
103008 |
| Tag Length |
17 |
| |
|
| Source name |
Cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: LNCaP
agent: R1881 androgen
|
| Biomaterial provider |
American Type Culture Collection
|
| Treatment protocol |
1 x 106 LNCaP cells were seeded in 10 cm-diameter dishes. The next day, cells were serum starved for 48 hours and then treated for 16 hours with vehicle (ethanol; final concentration 2.85 x 10%).
|
| Growth protocol |
LNCaP human prostate cancer cells were maintained in RPMI-1640 media supplemented with 10% v/v fetal bovine serum, 100 units/mL penicillin and 100 units/mL streptomycin.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using TRIZOL Reagent following the manufacturer’s instructions.
|
| |
|
| Description |
LongSAGE libraries were constructed with 5ug of total RNA using the Invitrogen I-SAGE Long kit.
|
| |
|
| Submission date |
Oct 02, 2009 |
| Last update date |
Oct 17, 2009 |
| Contact name |
Marianne D. Sadar |
| Organization name |
British Columbia Cancer Agency
|
| Department |
Genome Sciences Centre
|
| Street address |
675 W. 10th Ave
|
| City |
Vancouver |
| State/province |
British Columbia |
| ZIP/Postal code |
V5Z 1L3 |
| Country |
Canada |
| |
|
| Platform ID |
GPL1485 |
| Series (1) |
| GSE18401 |
Identification of novel androgen-responsive genes by sequencing of LongSAGE libraries |
|
