|
Status |
Public on Jun 17, 2021 |
Title |
GBM22 1 |
Sample type |
RNA |
|
|
Source name |
untreated GBM22
|
Organism |
Homo sapiens |
Characteristics |
cell type: GBM22 patient-derived xenograft cells group: untreated
|
Treatment protocol |
Alisertib-resistant cells were obtained by maintaining cells in DMEM+10%FBS+1uM Alisertib for 10 days.
|
Growth protocol |
Cells were maintained in DMEM+10%FBS.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated using miRNAeasy Mini Kit (QIAGEN 217004) according to the manufacturer's protocol.
|
Label |
biotin
|
Label protocol |
Biotin labeling was performed using the WT Plus reagent kit (Affymetrix, Santa Clara, CA) according to the manufacturer's protocol.
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|
|
Hybridization protocol |
The labeled, fragmented DNA was hybridized to the Affymetrix Human Gene 2.0 ST for 18 hours in a GeneChip Hybridization oven 640 at 45oC with rotation (60 rpm). The hybridized samples were washed and stained using an Affymetrix fluidics station 450.
|
Scan protocol |
After staining, microarrays were immediately scanned using an Affymetrix GeneArray Scanner 3000 7G Plus.
|
Data processing |
Raw Affymetrix CEL files were normalized to produce Entrez-Gene-identifier-specific expression values using the implementation of the Robust Multiarray Average (RMA) in the affy Bioconductor package (version 1.36.1), using R version 2.15.1 and the Brainarray hugene20sthsentrezgcdf R package (version 17.0.0).
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|
|
Submission date |
Jun 16, 2020 |
Last update date |
Jun 17, 2021 |
Contact name |
Boston University Microarray and Sequencing Resource |
E-mail(s) |
msrdata@bu.edu
|
Organization name |
Boston University
|
Department |
Microarray and Sequencing Resource
|
Street address |
72 East Concord Street, E631
|
City |
Boston |
State/province |
MA |
ZIP/Postal code |
02118 |
Country |
USA |
|
|
Platform ID |
GPL17930 |
Series (1) |
GSE152612 |
AURKA Inhibition Reprograms Metabolism dependent on PGC1α to Drive Synthetic Lethality with Fatty Acid Oxidation Inhibition in Glioblastoma |
|