|
| Status |
Public on May 17, 2010 |
| Title |
CHM050 |
| Sample type |
genomic |
| |
|
| Source name |
complete hydatidiform mole
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: complete hydatidiform mole
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted from complete hydatidiform mole tissues using the Qiagen DNA Mini Kit. DNA quality and quantity was assessed using a Nanodrop Spectrophotometer, PicoGreen and agarose gel electrophoresis.
|
| Label |
biotin
|
| Label protocol |
As per manufacturer (Affymetrix)
|
| |
|
| Hybridization protocol |
DNA was restriction digested, PCR amplified, fragmented, labeled and hybridized to each array according to the manufacturer's instructions.
|
| Scan protocol |
The arrays were washed using Affymetrix fluidics stations, and scanned using the Gene Chip Scanner 3000 7G.
|
| Description |
none
|
| Data processing |
CEL files were processed by Affymetrix Genotyping Console 3.0.1. Calls were generated using birdseed-v2 algorithm. Copy number analysis was performed using "Reference Model File Creation and Analysis" mode without with regional GC correction configuration.
|
| |
|
| Submission date |
Oct 20, 2009 |
| Last update date |
May 17, 2010 |
| Contact name |
Kenshi Hayashi |
| E-mail(s) |
hayashi.kenshi@gmail.com
|
| Phone |
+81-92-642-6171
|
| Organization name |
Kyushu University
|
| Department |
Medical Institute of Bioregulation
|
| Street address |
Maidashi 3-1-1, Higashi-ku
|
| City |
Fukuoka |
| ZIP/Postal code |
812-8582 |
| Country |
Japan |
| |
|
| Platform ID |
GPL6801 |
| Series (2) |
| GSE18642 |
Definitive SNP/CNV haplotype map of Asians determined using a collection of complete hydatidiform moles (Affymetrix) |
| GSE18701 |
Definitive SNP/CNV haplotype map of Asians determined using a collection of complete hydatidiform moles |
|
