|
| Status |
Public on Oct 30, 2009 |
| Title |
ODN/Pam 3 |
| Sample type |
RNA |
| |
|
| Source name |
2h treatment of MLE-15 cells
|
| Organism |
Mus musculus |
| Characteristics |
cell type: MLE-15
|
| Treatment protocol |
MLE-15 cells were treated with PBS, ODN2395 (0.4 ug), Pam2CSK4 (0.2 ug) or ODN2395+Pam2CSK4 for 2 hours. For each, a 20 ul volume of the treatment was added to a 500 ul volume of RPMI for each well of 6-well tissue culture plates.
|
| Growth protocol |
MLE-15s grown in monolayer to approximately 80% confluence in 6 well plates
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA extracted using Qiagen Rneasy per manufacturers instructions. cRNA amplification using Ambion total prep kit per manufacturers instructions.
|
| Label |
Cy3
|
| Label protocol |
cRNA was hybridized to BeadChips prior to labeling with Cy3-Streptavidin per Illumina instructions.
|
| |
|
| Hybridization protocol |
cRNA was hybridized to BeadChips per Illumina instructions.
|
| Scan protocol |
Chips were scanned using level 3 signal using a BeadStation 500 scanner.
|
| Description |
Combination treated
|
| Data processing |
Data was processed using Illumina BeadStudio v3. Matrix tables were generated both without normalization and with rank invariant normalization, using sham group as the reference group.
|
| |
|
| Submission date |
Oct 23, 2009 |
| Last update date |
Oct 29, 2009 |
| Contact name |
Scott E Evans |
| E-mail(s) |
seevans@mdanderson.org
|
| Organization name |
University of Texas-M.D. Anderson Cancer Center
|
| Department |
Pulmonary Medicine - Box 403
|
| Street address |
1400 Holcombe Blvd
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE18725 |
Gene expression in respiratory epithelial cells treated to induce resistance with synthetic TLR ligands |
|
