|
| Status |
Public on Jul 29, 2020 |
| Title |
TC5.P2 |
| Sample type |
genomic |
| |
|
| Source name |
TC PDX tumor (TC5), mouse passage 2
|
| Organism |
Homo sapiens |
| Characteristics |
gender: male
cell type: Testicular cancer (TC)
|
| Treatment protocol |
DNA and RNA from testicular cancer PDX tumours of different generations were isolated from snap frozen tumour tissue.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Simultaneous isolation of DNA and RNA from the same sample was done using the AllPrep DNA/RNA mini kit (Qiagen, Hilden, Germany).
|
| Label |
Cy3 and Cy5
|
| Label protocol |
DNA processing, tagging and hybridization to the CHIP were performed according to the manufacturer's protocol (Illumina).
|
| |
|
| Hybridization protocol |
DNA processing, tagging and hybridization to the CHIP were performed according to the manufacturer's protocol (Illumina).
|
| Scan protocol |
DNA processing, tagging and hybridization to the CHIP were performed according to the manufacturer's protocol (Illumina).
|
| Description |
TC5.F3_DNA
|
| Data processing |
Genomic DNA extracted from testicular cancer PDX tumors was genotyped using the Infinium Global Screening Array-24 v1.0 BeadChip (Illumina) with extra custom content.
Primary assessment and SNP call rate quality control of SNP intensity output files were performed using GenomeStudio software (Illumina).
|
| |
|
| Submission date |
Jul 28, 2020 |
| Last update date |
Jul 29, 2020 |
| Contact name |
Steven de Jong |
| Organization name |
University Medical Center Groningen
|
| Department |
Medical Oncology
|
| Street address |
Hanzeplein 1
|
| City |
Groningen |
| ZIP/Postal code |
9713 GZ |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL28939 |
| Series (2) |
| GSE155262 |
Establishment and characterization of testicular cancer patient-derived xenograft (PDX) models [SNP array] |
| GSE155277 |
Establishment and characterization of testicular cancer patient-derived xenograft (PDX) models |
|
