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Sample GSM473731 Query DataSets for GSM473731
Status Public on Feb 12, 2010
Title 4457278015_F
Sample type RNA
 
Source name C17.2 murine neural stem cells transduced with pBabe control retroviral vector
Organism Mus musculus
Characteristics cell type/line: C17.2
transduction: control pBabe retroviral vector
Extracted molecule total RNA
Extraction protocol TRIZOL followed by MagMAX96 for microarrays total RNA isolation kit (Ambion)
Label biotin
Label protocol 200 ng total RNA were processed according to Illumina TotalPrep RNA amplification kit (Ambion)
 
Hybridization protocol According to Illumina whole-genome gene expression direct hybridization assay guide
Scan protocol The arrays were scanned using an iScan (Illumina). Images were segmented and quantified by BeadStudio (Illumina).
Description Control retrovirus transduction of C17.2 murine neural stem cells. 200 ng of total RNA were hybridized in Illumina MouseWG-6v2 expression arrays.
Data processing BeadStudio-based background subtraction; followed by variance stabilization transformation and robust spline normalization as implemented in lumi R package (Bioconductor)
 
Submission date Nov 20, 2009
Last update date Feb 11, 2010
Contact name Mariano Javier Alvarez
Organization name Columbia University
Department Systems Biology
Lab Systems Biology
Street address 1130 St. Nicholas ave.
City New York
State/province NY
ZIP/Postal code 10032
Country USA
 
Platform ID GPL6887
Series (2)
GSE19113 A transcriptional module initiates and maintains mesenchymal transformation in brain tumors [mouse data]
GSE20281 The transcriptional network for mesenchymal transformation of brain tumours

Data table header descriptions
ID_REF Probe identifiers, each of which is unique to a specific probe.
VALUE Signal intensity level for each probe.
DETECTION Detection p-value as estimated by BeadStudio.

Data table
ID_REF VALUE DETECTION
ILMN_2417611 6.89783014754671 0.7403846
ILMN_2762289 6.96279852901258 0.2553419
ILMN_2896528 9.56915757002075 0
ILMN_2721178 8.18878653845074 0
ILMN_2458837 6.90423958922692 0.6720086
ILMN_3033922 8.80728714894246 0
ILMN_3092673 9.68400153612456 0
ILMN_1230777 9.23242077010016 0
ILMN_1246069 9.70382099864628 0
ILMN_1232042 6.88751108880759 0.8514957
ILMN_1243193 8.92653978616666 0
ILMN_2524361 6.94947341939534 0.3119658
ILMN_1242440 6.87717756245548 0.9123932
ILMN_1233188 6.89556915577436 0.7681624
ILMN_2543688 6.91219153914984 0.590812
ILMN_1259789 7.0931252812768 0.03632479
ILMN_2816356 7.28061645650497 0.002136752
ILMN_1224596 7.32392620202388 0.001068376
ILMN_1233643 7.01096236334503 0.1442308
ILMN_2808939 8.54608462240132 0

Total number of rows: 45281

Table truncated, full table size 1683 Kbytes.




Supplementary file Size Download File type/resource
GSM473731.txt.gz 741.6 Kb (ftp)(http) TXT
Processed data included within Sample table

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