|
| Status |
Public on Feb 12, 2010 |
| Title |
4457278021_A |
| Sample type |
RNA |
| |
|
| Source name |
C17.2 murine neural stem cells transduced with a constitutively active mutant of STAT3 cloned in a retroviral vector
|
| Organism |
Mus musculus |
| Characteristics |
cell type/line: C17.2
transduction: STAT3C retroviral vector
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TRIZOL followed by MagMAX96 for microarrays total RNA isolation kit (Ambion)
|
| Label |
biotin
|
| Label protocol |
200 ng total RNA were processed according to Illumina TotalPrep RNA amplification kit (Ambion)
|
| |
|
| Hybridization protocol |
According to Illumina whole-genome gene expression direct hybridization assay guide
|
| Scan protocol |
The arrays were scanned using an iScan (Illumina). Images were segmented and quantified by BeadStudio (Illumina).
|
| Description |
STAT3C retrovirus transduction of C17.2 murine neural stem cells. 200 ng of total RNA were hybridized in Illumina MouseWG-6v2 expression arrays.
|
| Data processing |
BeadStudio-based background subtraction; followed by variance stabilization transformation and robust spline normalization as implemented in lumi R package (Bioconductor)
|
| |
|
| Submission date |
Nov 20, 2009 |
| Last update date |
Feb 11, 2010 |
| Contact name |
Mariano Javier Alvarez |
| Organization name |
Columbia University
|
| Department |
Systems Biology
|
| Lab |
Systems Biology
|
| Street address |
1130 St. Nicholas ave.
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
| |
|
| Platform ID |
GPL6887 |
| Series (2) |
| GSE19113 |
A transcriptional module initiates and maintains mesenchymal transformation in brain tumors [mouse data] |
| GSE20281 |
The transcriptional network for mesenchymal transformation of brain tumours |
|
