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Sample GSM473754 Query DataSets for GSM473754
Status Public on Feb 12, 2010
Title 4457278013_E
Sample type RNA
 
Source name Primary murine neural stem cells (NCS) isolated from E13.5 mouse telencephalon, transduced with STAT3 and CEBPB retroviral vectors.
Organism Mus musculus
Characteristics cell type/line: NSC
transduction: STAT3C and CEBPB retroviral vectors
Extracted molecule total RNA
Extraction protocol TRIZOL followed by MagMAX96 for microarrays total RNA isolation kit (Ambion)
Label biotin
Label protocol 200 ng total RNA were processed according to Illumina TotalPrep RNA amplification kit (Ambion)
 
Hybridization protocol According to Illumina whole-genome gene expression direct hybridization assay guide
Scan protocol The arrays were scanned using an iScan (Illumina). Images were segmented and quantified by BeadStudio (Illumina).
Description STAT3C and CEBPB retrovirus transduction of murine NSCs. 200 ng of total RNA were hybridized in Illumina MouseWG-6v2 expression arrays.
Data processing BeadStudio-based background subtraction; followed by variance stabilization transformation and robust spline normalization as implemented in lumi R package (Bioconductor)
 
Submission date Nov 20, 2009
Last update date Feb 11, 2010
Contact name Mariano Javier Alvarez
Organization name Columbia University
Department Systems Biology
Lab Systems Biology
Street address 1130 St. Nicholas ave.
City New York
State/province NY
ZIP/Postal code 10032
Country USA
 
Platform ID GPL6887
Series (2)
GSE19113 A transcriptional module initiates and maintains mesenchymal transformation in brain tumors [mouse data]
GSE20281 The transcriptional network for mesenchymal transformation of brain tumours

Data table header descriptions
ID_REF Probe identifiers, each of which is unique to a specific probe.
VALUE Signal intensity level for each probe.
DETECTION Detection p-value as estimated by BeadStudio.

Data table
ID_REF VALUE DETECTION
ILMN_2417611 6.8641468937766 0.8931624
ILMN_2762289 6.86150379491552 0.9038461
ILMN_2896528 10.5206289736997 0
ILMN_2721178 8.8426496519751 0
ILMN_2458837 6.91110943572173 0.5715812
ILMN_3033922 9.38193315039013 0
ILMN_3092673 10.5143546497356 0
ILMN_1230777 9.83362707262967 0
ILMN_1246069 9.85917212793303 0
ILMN_1232042 6.92757315376829 0.448718
ILMN_1243193 8.43906637696357 0
ILMN_2524361 6.92586455407606 0.4594017
ILMN_1242440 7.00286503601397 0.04807692
ILMN_1233188 6.86008013146336 0.9070513
ILMN_2543688 7.0040191549731 0.04807692
ILMN_1259789 7.10342133824643 0.001068376
ILMN_2816356 7.11582188769831 0.001068376
ILMN_1224596 7.40101964564305 0
ILMN_1233643 6.90990549753163 0.5747863
ILMN_2808939 8.82860464903347 0

Total number of rows: 45281

Table truncated, full table size 1667 Kbytes.




Supplementary file Size Download File type/resource
GSM473754.txt.gz 731.4 Kb (ftp)(http) TXT
Processed data included within Sample table

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