|
| Status |
Public on Sep 10, 2023 |
| Title |
ESCC_rep1 [miRNA] |
| Sample type |
RNA |
| |
|
| Source name |
esophageal squamous cell carcinoma tissue
|
| Organism |
Homo sapiens |
| Characteristics |
gender: female
age: 72
|
| Treatment protocol |
ESCC tissues and normal adjacent tissues from 6 samples
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was prepared using the TRIzol reagent following the manufacturer's recommendations. RNA was quantified using a NanoDrop-1000 spectrophotometer and RNA integrity was determined by 1% formaldehyde denaturing gel electrophoresis.
|
| Label |
Cy3
|
| Label protocol |
The miRNAs were labeled using the Agilent miRNA labeling reagent. Total RNA (200 ng) was dephosphorylated and ligated with pCp-Cy3, the labeled RNA was purified and hybridized to miRNA arrays.
|
| |
|
| Hybridization protocol |
According to the Agilent miRNA Microarray Protocol (Agilent Technologies).
|
| Scan protocol |
After hybridization and washes, slides were scanned immediately in Agilent Microarray Scanner with Surescan High Resolution Technology (Agilent Technologies, Santa Clara, CA).
|
| Description |
Gene expression of esophageal squamous cell carcinoma tissue
|
| Data processing |
Feature Extraction v10.10 (Agilent Technologies, CA) software was used to extract all features of the data obtained from the scanned images and Bioconductor software was used to analyze the raw data, which are normalized by percentile normalization.
|
| |
|
| Submission date |
Sep 11, 2020 |
| Last update date |
Sep 10, 2023 |
| Contact name |
Yaowen Zhang |
| E-mail(s) |
zhangyaowen621@126.com
|
| Phone |
+8615837207287
|
| Organization name |
Anyang Cancer Hospital
|
| Department |
Radiotherapy
|
| Street address |
No. 1 Huanbin North Road, Beiguan District
|
| City |
Anyang |
| State/province |
Henan |
| ZIP/Postal code |
455000 |
| Country |
China |
| |
|
| Platform ID |
GPL25134 |
| Series (2) |
| GSE157805 |
microRNA microarray profile analysis in esophageal squamous cell carcinoma(ESCC) and adjacent normal tissues |
| GSE157808 |
Analysis in esophageal squamous cell carcinoma(ESCC) and adjacent normal tissues |
|
