|
| Status |
Public on Sep 23, 2020 |
| Title |
Wild type_ICH_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
HSCs
|
| Organism |
Mus musculus |
| Characteristics |
tissue: bone marrow
genotype: Wild type
agent: ICH
Sex: Male
|
| Extracted molecule |
total RNA |
| Extraction protocol |
HSC were sorted from femur bone marrow of ICH and sham mice at day 3 after surgery. Cells were directly sorted into Trizol LS (ThermoFisher)-containing tubes, and total RNA was then extracted.
|
| Label |
n/a
|
| Label protocol |
n/a
|
| |
|
| Hybridization protocol |
Each mRNA was hibridized with two ~50 base probes in the hybidize solution. The reporter probe carries the signal, the capture probe allows the complex to be immobilized for data collection.
|
| Scan protocol |
After hybridization, excess probes are removed and the probe/target complexes aligned and immobilized in the nCounter Cartrige. Sample cartriges are placed in the Digital Analyzer. Color codes on the surface of the cartrige are counted and tabulated for each target mRNA.
|
| Description |
HSCs were flow cytometric sorted from bone marrow of mice
|
| Data processing |
Data were processed on the nSolver software. Data were normalized to the 14 reference genes including positive and negative controls and housekeeping genes probes using nSolver software
|
| |
|
| Submission date |
Sep 22, 2020 |
| Last update date |
Sep 23, 2020 |
| Contact name |
Kaibin Shi |
| E-mail(s) |
shikaibin1992@outlook.com
|
| Phone |
18702209661
|
| Organization name |
Tianjin Medical University
|
| Department |
Neurology
|
| Street address |
154 Anshan Rd, Heping District
|
| City |
Tianjin |
| ZIP/Postal code |
300052 |
| Country |
China |
| |
|
| Platform ID |
GPL25266 |
| Series (1) |
| GSE158332 |
The effects of beta-3 adrenergic innervation on bone marrow cells |
|
