|
| Status |
Public on Jan 04, 2013 |
| Title |
cerebellum_individual 2 |
| Sample type |
RNA |
| |
|
| Source name |
Adult human cerebellum:individual 2
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Adult human cerebellum
gender: male
age: 45
|
| Treatment protocol |
All human postmortem brain tissue samples were obtained from the NICHD Brain and Tissue Bank for Developmental Disorders (NICHDBB)(Baltimore, MD, USA). All subjects were defined as normal controls by forensic pathologists at the NICHDBB. No subjects with prolonged agonal state were used.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from 50mg frozen sample using a mirVana RNA Isolation Kit (Ambion, AM1560), according to the manufacturer’s instructions.The quality of each sample was checked by using an Agilent 2100 Bioanalyzer.
|
| Label |
Cy3
|
| Label protocol |
For each sample, 100ng total of RNA was dephosphorylated and ligated with pCp-Cy3, following the manufacturer’s instructions, as described in the miRNA Complete Labeling and Hybridization Kit (Agilent Technologies).
|
| |
|
| Hybridization protocol |
The labeled RNA was purified and hybridized to Agilent Human miRNA Microarray following the manufacturer’s instructions
|
| Scan protocol |
The slides were scanned with the Agilent Microarray Scanner using one color scan setting for array slides.
|
| Description |
none
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software (Agilent) to obtain TotalGeneSignal. microRNAs with features in SystematicName column begining with hsa- and in column IsGeneDetected as 1 remained, others were removed. This resulted in detecting expression of 429 human miRNA in at least one of the 9 samples studied.
|
| |
|
| Submission date |
Dec 15, 2009 |
| Last update date |
Jan 04, 2013 |
| Contact name |
Ning Fu |
| E-mail(s) |
nfu@sibs.ac.cn
|
| Organization name |
Chinese Academy of Sciences
|
| Department |
Shanghai Institute for Biological Sciences
|
| Lab |
Key Laboratory of Systems Biology
|
| Street address |
320# Yue-Yang Road
|
| City |
Shanghai |
| ZIP/Postal code |
200031 |
| Country |
China |
| |
|
| Platform ID |
GPL8227 |
| Series (1) |
| GSE19505 |
Gene expression data from postnatal cerebellum, prefrontal cortex and liver: comparison on tissue differences (miRNA) |
|
