|
| Status |
Public on Dec 19, 2009 |
| Title |
end2_3 |
| Sample type |
RNA |
| |
|
| Source name |
cell line
|
| Organism |
Mus musculus |
| Characteristics |
strain: p19
cell type: embryonal carcinoma cell line
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Thermo Scientific nanodrop 1000.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol (58 degrees for 16-20 hours).
|
| Scan protocol |
Standard Illumina scanning protocol.
|
| Description |
replicate 3
|
| Data processing |
The data was normalized using LumiExpresso_(_) function of the lumi Bioconductor package in R (www.bioconductor.org).
|
| |
|
| Submission date |
Dec 18, 2009 |
| Last update date |
Dec 18, 2009 |
| Contact name |
Ann Foley |
| E-mail(s) |
acf2002@med.cornell.edu
|
| Phone |
212-746-1361
|
| Fax |
212-746-6669
|
| Organization name |
Weill Cornell Medical College
|
| Department |
Medicine
|
| Lab |
Cardiology
|
| Street address |
510 E70th Street
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10021 |
| Country |
USA |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE19564 |
Comparative Analysis of Extraembryonic Endoderm Cells with Cardiac Inducing Ability |
|
