tissue: Total Aorta genotype: apoE KO activation agent: treated time: 7 days
Extracted molecule
total RNA
Extraction protocol
Total RNA was extracted with RNeasy Mini Kit followed by DNase I treatment.
Label
SYBR Green
Label protocol
:Quantitative real-time PCR were performed (Light Cycler 480, Roche) with 45 cycles at 94 oC for 15 seconds and 60 oC for 60 seconds.
Hybridization protocol
n/a
Scan protocol
n/a
Description
Treated SAMPLE 4
Data processing
The normalization and all the data analysis were performed according to the manufacturers instructions using their web-based software package: http://sabiosciences.com/pcrarraydataanalysis.php For the normalization it uses the average of five housekeeping genes: GUSB, HPRT , HSP90AB1, GAPDH, ACTB. Target gene signals normalized to housekeeping genes; 2^-deltaCt, where deltaCt = (Ct_Target − Ct_HKG)] The web-based software package automatically performs all deltadeltaCt based fold-change calculations from the uploaded raw threshold cycle data. Fold Change file reports test/control (i.e., Scramble/LR12) ratios.
