|
| Status |
Public on Feb 15, 2010 |
| Title |
Mouse No Cldn16 B9 458 |
| Sample type |
RNA |
| |
|
| Source name |
genotype Cldn16 +/+, renal tissue
|
| Organism |
Mus musculus |
| Characteristics |
gender: female
age: juvenile, 8 weeks (P58)
strain: Sv129 / C57BL/6N mixed background
genotype/variation: Cldn16 +/+
tissue type: renal tissue
|
| Growth protocol |
Animals were of the following genetic background: 129S7/SvEvBrd-Hprtb-m2, F2, 1 backross to C57BL/6N. Mice were weaned at the age of 4 weeks, and afterwards supplied with standard nutrition until sacrification.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Poles were removed from freshly dissected kidneys, followed by immediate snap-freezing.
Total RNA was extracted with Trizol reagent, followed by DNAse treatment and purification via RNEasy columns. RNA purity and concentration was assessed with a Bioanalyzer.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared with the AMIL1791 Illumina® TotalPrep™ RNA Amplification Kit.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
4466975077_b
|
| Data processing |
The data were normalised using quantile normalisation with BeadStudio Software. Data were exported to Microsoft Excel for applying student´s t-test on analysis groups of Cldn16 -/- and Cldn16 +/+ animals. Data with a p-value of < 0.05 were considered as significant.
|
| |
|
| Submission date |
Jan 20, 2010 |
| Last update date |
Feb 15, 2010 |
| Contact name |
Constanze Will |
| Organization name |
Charité
|
| Department |
CCR
|
| Street address |
Hessische Str. 3 - 4
|
| City |
Berlin |
| ZIP/Postal code |
10115 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE19961 |
Gene Expression Profile in kidneys from Cldn16 +/+, +/-, -/- female mice |
|
