|
| Status |
Public on Dec 28, 2022 |
| Title |
7 days post bolus infection wild-type mouse sample 04 |
| Sample type |
RNA |
| |
|
| Source name |
6-8 week old female C57BI/6 mouse host, 200 H. polygyrus larvae
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BI/6
age: 6-8 week old
Sex: female
infection: 200 H. polygyrus larvae
infection type: Bolus
time: 7 days post infection
tissue: worm-induced intestinal granuloma in mouse
|
| Treatment protocol |
Mice were infected with H. polygyrus larvae according to infection regime group. After 7 or 21 days, granulomas in the small intestine were isolated for RNA extraction.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using a phenol-chloroform extraction (Trizol) and stored at -80C.
|
| Label |
N/A
|
| Label protocol |
Labeling was performed according to manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Hybridization was performed according to manufacturer's instructions.
|
| Scan protocol |
nCounter Sprint profiler was used for scanning and image acquisition
|
| Description |
D7_WT_Bolus_04
|
| Data processing |
nanoString data were analyzed in R. Raw counts were normalized using NanoStringNorm (using the positive control, negative control, and Eif2b4, Polr1b, and Edc3 housekeeping probes)
|
| |
|
| Submission date |
Jan 05, 2021 |
| Last update date |
Dec 28, 2022 |
| Contact name |
Constance Finney |
| Organization name |
University of Calgary
|
| Street address |
2500 University Drive NW
|
| City |
Calgary |
| State/province |
Alberta |
| ZIP/Postal code |
T2N 1N4 |
| Country |
Canada |
| |
|
| Platform ID |
GPL25266 |
| Series (1) |
| GSE164319 |
NanoString analysis of mouse granulomas induced by H. polygyrus infection |
|
