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Sample GSM501382 Query DataSets for GSM501382
Status Public on Jan 27, 2010
Title 50% C2 blasts_rep2
Sample type RNA
 
Source name C2C12 cell line
Organism Mus musculus
Characteristics cell line: C2C12
cell type: myoblast
confluence: 50%
Growth protocol C2C12 myoblasts were cultured in DMEM supplemented with 10% BCS. If needed, 100% confluent myoblasts were induced to differentiate into myotubes in DMEM supplemented with 2% heat-inactive horse serum, 10ug/ml insulin, and 10ug/ml transferrin
Extracted molecule total RNA
Extraction protocol total RNA was extracted with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description replicate 2
Data processing The data were normalized and summarized using RMA in the lumi package of Bioconductor
 
Submission date Jan 27, 2010
Last update date Jan 27, 2010
Contact name Yi Cao
E-mail(s) ycao@fhcrc.org
Phone 206-667-5278
Organization name Fred Hutchinson CRC
Department Human Biology
Lab Tapscott
Street address 1100 Fairview Ave. N
City Seatttle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL6887
Series (1)
GSE20059 Genome-wide analysis of gene expression during differentiation in C2C12 cells

Data table header descriptions
ID_REF
VALUE log2-transformed, quantile normalized signal intensity
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_2417611 6.511 0.771
ILMN_2762289 6.604 0.363
ILMN_2896528 8.440 0.000
ILMN_2721178 7.286 0.000
ILMN_2458837 6.457 0.953
ILMN_3033922 9.794 0.000
ILMN_3092673 11.573 0.000
ILMN_1230777 11.034 0.000
ILMN_1246069 11.453 0.000
ILMN_1232042 6.588 0.411
ILMN_1243193 10.504 0.000
ILMN_2524361 6.726 0.118
ILMN_1242440 6.582 0.431
ILMN_1233188 7.164 0.000
ILMN_2543688 8.461 0.000
ILMN_1259789 6.974 0.013
ILMN_2816356 7.013 0.005
ILMN_1224596 7.219 0.000
ILMN_1233643 6.658 0.236
ILMN_2808939 10.119 0.000

Total number of rows: 45281

Table truncated, full table size 1109 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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