|
| Status |
Public on Dec 30, 2010 |
| Title |
Huh7 |
| Sample type |
RNA |
| |
|
| Source name |
human liver cancer cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: human liver cancer cell line
cell line: Huh7
|
| Growth protocol |
The hepatoma cell lines SK-HEP-1 [American Type Culture Collection (ATCC) number HTB-52], HepG2( ATCC number HB-8065), Hep 3B( ATCC number HB-8064), Huh7, MHCC97-H and MHCC97-L, SMMC-7721 (China Center for Type Culture Collection, resource number 3115CNCB00263), and HEK293 and HeLa cell lines were cultured and maintained in Dulbecco’s modified Eagle medium (DMEM) or modified Eagle's minimal essential medium (MEM), supplemented with 100 ml/l fetal calf serum (FCS, Gibco, USA), 100 IU/ml penicillin, 100μg /ml streptomycin at 37 ℃ in a humidified incubator with 5% CO2 (see Supplementary Figure S1). Normal primary human hepatocytes (PHHC-1， PHHC-2， PHHC-3) were isolated from 3 normal liver tissues adjacent to hepatic hemangioma under sterile conditions using a modified four-step retrograde perfusion technique as reported elsewhere.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The MirVanaTM miRNA Isolation Kit (Ambion, AM1560) was used to isolate total RNA (including miRNA) from all cell lines according to the manufacturer’s protocol.
|
| Label |
Cy3
|
| Label protocol |
see manufacturer's web site at http://www.agilent.com/
|
| |
|
| Hybridization protocol |
see manufacturer's web site at http://www.agilent.com/
|
| Scan protocol |
Images were scanned with the Agilent microarray scanner and the results were obtained by Agilent feature extraction software, Scan resolution 5μm, PMT 100%，5%
|
| Description |
US80803205_251911811853_S01_2_3.txt
|
| Data processing |
The scanned images were analyzed with Agilent Feature Extraction Software, the raw intensity data were quantile normalized and further analyzed using GeneSpring10.0 software.
|
| |
|
| Submission date |
Jan 28, 2010 |
| Last update date |
Dec 30, 2010 |
| Contact name |
Xing-Xing He |
| E-mail(s) |
healthhe@hotmail.com
|
| Phone |
86-27-69378506
|
| Organization name |
Tongji Hospital
|
| Department |
Institute of Liver and Gastrointestinal Diseases
|
| Lab |
Institute of Liver and Gastrointestinal Diseases
|
| Street address |
1095# Jie-Fang Avenue
|
| City |
Wuhan |
| State/province |
Hubei |
| ZIP/Postal code |
430030 |
| Country |
China |
| |
|
| Platform ID |
GPL8227 |
| Series (1) |
| GSE20077 |
Aberrantly microRNA profiling in cancerous hepatocytes versus normal primary hepatocytes |
|
