|
| Status |
Public on Jan 28, 2021 |
| Title |
RMac transferred into clodronate-depleted recipients D32 |
| Sample type |
RNA |
| |
|
| Source name |
Peritoneal cavity
|
| Organism |
Mus musculus |
| Characteristics |
donor treatment: Naive
host treatment: Clodronate-depleted
matrix: 2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
For each of the indicated donor populations 5000 cells were sorted into 2µl of RLT (QIAGEN). Immediately after cells where: centrifuged at maximum speed 15 seconds, vortexed for 10 seconds and again centrifuged at maximum speed 15 seconds. Cells where stored at -80degrees C until analysis of the lysate using the nCounter Myeloid innate immunity panel.
|
| Label |
n.a.
|
| Label protocol |
n.a.
|
| |
|
| Hybridization protocol |
n.a.
|
| Scan protocol |
n.a.
|
| Description |
clodronate-depleted recipient
|
| Data processing |
Data were imported into nSolver 4.0 software (Nanostring Technologies). Data were annotated by cell-type for analysis (RMac, RMacZ10, IMacZ10).Grouped data were then analysed using the advanced analysis nSolver package according to the manufacturers instructions. Importantly, donor populations transferred into mirroring (D1-D17) or clodronate-depleted (D20-D37) recipients were obtained on different days and were analysed separately.
|
| |
|
| Submission date |
Jan 18, 2021 |
| Last update date |
Jan 28, 2021 |
| Contact name |
Pieter Adriaan Louwe |
| Organization name |
University of Edinburgh
|
| Department |
Centre for Inflammation Research
|
| Lab |
Jenking laboratory
|
| Street address |
47 Little France Crescent
|
| City |
Edinburgh |
| State/province |
Scotland |
| ZIP/Postal code |
EH15 1JA |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL25266 |
| Series (1) |
| GSE165036 |
Nanostring analysis of macrophage subsets following zymosan-induced peritonitis |
|
