|
Status |
Public on Jun 24, 2010 |
Title |
Prostate tumor PCA0093 miRNA |
Sample type |
RNA |
|
|
Source name |
human prostate
|
Organism |
Homo sapiens |
Characteristics |
sample id: PCA0093 tissue: Prostate disease status: prostate cancer tumor type: Primary tumor biopsy_gleason_grade: 6 clint_stage: T1C pathological_stage: T2B
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolaged using TRIzol@Reagent Total RNA Isoloation (Invitrogen), quantified with Thermo Scientific NanoDrop 1000 Spectrophotometer, and quality controlled with RNA 6000 picoAssay and Bioanalyzer 2100 (Agilent).
|
Label |
Cy3
|
Label protocol |
200ng of total RNA was labeled using the miRNA Complete Labeling and Hybridization Kit (Agilent)
|
|
|
Hybridization protocol |
Per manufacturer's instructions
|
Scan protocol |
Per manufacturer's instructions
|
Description |
microRNA expression profiling
|
Data processing |
Images were quantified using Agilent Feature Extraction version 9.5 and data was normalized with between-array variance stabilization normalization (VSN) after excluding any microRNA not present in >80% of the samples profiled
|
|
|
Submission date |
Mar 24, 2010 |
Last update date |
Jun 15, 2010 |
Contact name |
Barry Stephen Taylor |
Organization name |
Memorial Sloan-Kettering Cancer Center
|
Street address |
1275 York Avenue, Box #460
|
City |
New York |
State/province |
NY |
ZIP/Postal code |
10065 |
Country |
USA |
|
|
Platform ID |
GPL8227 |
Series (2) |
GSE21032 |
Integrative genomic profiling of human prostate cancer |
GSE21036 |
MicroRNA expression data for human primary and metastatic prostate cancer samples and control normal adjacent benign prostate |
|