developmental stage: mature mushrooms strain: Dikaryon, resulting from a cross of the co-isogenic monokaryotic strains 4-40 (CBS 340.81) and 4-39 (CBS 341.81)
Growth protocol
Mature mushrooms of 3 days old were picked from dikaryotic cultures that had grown for 8 days in the light.
Extracted molecule
total RNA
Extraction protocol
Total RNA was isolated using TRIzol (Invitrogen) and purified over a column (Qiagen RNeasy Mini). PolyA RNA was isolated using oligo-dT beads.
Description
Anchoring enzyme: DpnII
Data processing
Tags of 16 bp were identified using custom scripts. Note that the restriction site of DpnII (GATC) is not included in these 16 bp.
