|
| Status |
Public on Jul 28, 2021 |
| Title |
DS-MHCII-CD64+Ly6C-low |
| Sample type |
RNA |
| |
|
| Source name |
Mouse conjuctiva
|
| Organism |
Mus musculus |
| Characteristics |
experimental group: Dessication stress
Sex: Female
age: 10-weeks
tissue: conjuctiva
cell type: DS-MHCII-CD64+Ly6C-low
|
| Treatment protocol |
Mice were either kept at normal condition or subjected to dessication stress under the standard protocol
|
| Growth protocol |
Mice were purchased from Jackson lab and maintained at BCM animal facilities until use
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Immune cells were sorted from the conjuctiva from each group and the RNA was extracted using the standard protocol provided by the manufactorer, we use the Qiagen RNA preparation kit
|
| Label |
biotin
|
| Label protocol |
The samples were labeled according to the condition in which the mice were kept and according to the gating strategy
|
| |
|
| Hybridization protocol |
One hundred nanograms of total RNA were hybridized with the Nanostring Technologies nCounter Gene Expression Mouse Myeloid Innate Immunity Panel codeset (NS_MM_Myeloid_V2.0+Pls) containing 770 Unique pairs of 35-50bp reporter probes, 12 custom probe pairs and biotin-labeled capture probes, including internal refereance controls. overnight hybridization occured for 20hours at 65C.
|
| Scan protocol |
The cartridge containing immobilized sample was transferred to the nCounter Digital Analyzer (Software v3.0.1.4) and scanned at 555 field of view (FOV).
|
| Description |
Sorted Cells for NanoString
MWF-DS4
|
| Data processing |
Data was processed using the nCounter Software nSolver 4.0
|
| |
|
| Submission date |
Jun 08, 2021 |
| Last update date |
Jul 28, 2021 |
| Contact name |
Jahan Alam |
| E-mail(s) |
jahan.Alam@bcm.edu
|
| Phone |
4026867021
|
| Organization name |
Baylor College of Medicine, Ocular-surface Center Lab
|
| Department |
Ophthalmology
|
| Lab |
Dr Steve Pflugfelder Lab
|
| Street address |
One Baylor Plaza, MS: BCM-706, Room C 505D,, MS: BCM-706, Room C 505D,
|
| City |
Houston |
| State/province |
Texas |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL25266 |
| Series (1) |
| GSE176390 |
Desiccating stress triggers conjunctival monocyte to macrophage cascade - Implications for Sjögren Syndrome keratoconjunctivitis Desiccation induced monocyte cascade |
|
