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Status |
Public on Jun 10, 2021 |
Title |
HNE-1 cells, NCsh |
Sample type |
SRA |
|
|
Source name |
KIF2C negative control cell
|
Organism |
Homo sapiens |
Characteristics |
cell line: HNE-1 genotype: KIF2C negative control cell
|
Treatment protocol |
HNE-1 cells were infected with the NCsh and KIF2Csh lentivirus particles with 5μg/mL polybrene for forty-eight hours
|
Growth protocol |
HNE-1 cells were cultured in RPMI 1640 medium containing 50 units/mL penicillin, 50 mg/mL streptomycin, and 10% (v/v) FBS
|
Extracted molecule |
total RNA |
Extraction protocol |
The KIF2C knockdown cells and its corresponding negative control cells were collected, total RNA was extracted, and cDNA libraries were then constructed and subjected to RNA-seq analysis RNA libraries were prepared for sequencing using standard MGI-G400/MGI-2000 protocols
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|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
MGISEQ-2000RS |
|
|
Data processing |
Bowtie2 was used to align raw data The bam files was quantify by RSEM Genome_build: mm9 Supplementary_files_format_and_content: genes_fpkm_expression.xlsx
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|
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Submission date |
Jun 09, 2021 |
Last update date |
Jun 10, 2021 |
Contact name |
Chuntao Tao |
Organization name |
Chongqing Medical University
|
Street address |
China
|
City |
Chongqing |
State/province |
State... |
ZIP/Postal code |
408500 |
Country |
China |
|
|
Platform ID |
GPL30209 |
Series (1) |
GSE176471 |
Cell cycle dysregulation with overexpression of KIF2C/MCAK is a critical event in nasopharyngeal carcinoma |
|
Relations |
BioSample |
SAMN19643024 |
SRA |
SRX11099999 |