|
| Status |
Public on Feb 14, 2022 |
| Title |
Xenograft-LuCaP35CR-T_tumor1 |
| Sample type |
SRA |
| |
|
| Source name |
xenograft
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: patient derived xenografts
treatment: 40mg/kg Depo-testosterone (daily) and sodium D-lactate (daily)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Rneasy Mini Kit (QIAGEN) following manufacturer’s protocol.
RNA-seq libraries of xenograft C4-2-tet-shRB (without Dox) were performed by using TruSeq® Strnd Total RNA LT (Illumina). RNA-seq libraries of the rest of samples were performed by using TruSeq® Stranded mRNA (Illumina).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
NextSeq 2000 |
| |
|
| Description |
Xenograft_LuCaP35CR_samples_count_table.txt
|
| Data processing |
The sequence quality of raw reads was checked by FastQC (version 0.11.9).
Raw reads were aligned to the human reference genome (hg19) using STAR (version 2.7.2) followed by counting with featureCounts (version 1.6.4) from GRCh37 Ensembl reference.
Genome_build: hg19
Supplementary_files_format_and_content: txt file forraw counts of sequence reads
|
| |
|
| Submission date |
Jul 07, 2021 |
| Last update date |
Feb 14, 2022 |
| Contact name |
Changmeng Cai |
| E-mail(s) |
changmeng.cai@umb.edu
|
| Organization name |
University of Massachusetts Boston
|
| Street address |
100 William T Morrissey Blvd
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02125 |
| Country |
USA |
| |
|
| Platform ID |
GPL30173 |
| Series (2) |
| GSE179687 |
CDK4/6 inhibition enhances the tumor-suppressive activity of high-dose androgens in castration-resistant prostate cancer [RNA-Seq] |
| GSE179688 |
CDK4/6 inhibition enhances the tumor-suppressive activity of high-dose androgens in castration-resistant prostate cancer |
|
| Relations |
| BioSample |
SAMN20109796 |
| SRA |
SRX11375843 |
