|
| Status |
Public on Oct 11, 2021 |
| Title |
Bmi1_Doc1 |
| Sample type |
SRA |
| |
|
| Source name |
Germinal Center B cells
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57BL/6
genotype/variation: Bmi1 conditional know-out in B cells
treatment: LCMV Docile
cell type: Germinal Center B cells
|
| Treatment protocol |
C57BL/6 mice were either treated with LCMV WE or Docile for 14 days. Germinal Center B cells were sort-purified from total splenocytes.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Snap frozen cell pellets were lysed and total RNA was isolated through the Rneasy Micro purification Kit (QIAGEN)
RNA libraries were prepared for sequencing using MGIEasy RNA Directional Library prep kit
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
MGISEQ-2000RS |
| |
|
| Data processing |
Alignment ti the mm10 refseq using Subread library
Total read counts using FeatureCounts
Differential expressed genes performing the DeSeq2 tool
Genome_build: mm10
Supplementary_files_format_and_content: CSV file containing: normalised counts, differential expressed genes, p values, FDR, Average expression, FClog
|
| |
|
| Submission date |
Aug 20, 2021 |
| Last update date |
Oct 11, 2021 |
| Contact name |
Andrea Di Pietro |
| E-mail(s) |
dipi.andrea@gmail.com
|
| Phone |
+610450240751
|
| Organization name |
Monash University
|
| Department |
Biochemistry and Molecular Biology
|
| Lab |
B cells, Antibody, Memory Laboratory
|
| Street address |
15 Innovation Walk, Monash University
|
| City |
Clayton |
| State/province |
VIC |
| ZIP/Postal code |
3800 |
| Country |
Australia |
| |
|
| Platform ID |
GPL30215 |
| Series (2) |
| GSE163365 |
Targeting BMI-1 in B cells restores effective humoral immune responses and controls chronic viral infection |
| GSE182491 |
Gene expression profiles in GC B cells of wild-type and Bmi1 cKO mice during acute or chronic viral infection |
|
| Relations |
| BioSample |
SAMN20865812 |
| SRA |
SRX11832750 |
