|
| Status |
Public on Jun 18, 2010 |
| Title |
ADSC-vector_adipogenic induction media-treated_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
ADSC-vector, adipogenic induction media-treated
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: adipose
cell type: adipose-derived stem cells (ADSCs)
transduced gene: control vector
treatment: adipogenic-induction media
|
| Treatment protocol |
For adipocyte differentiation, 2 day post-confluent cells were treated with differentiation-inducing medium containing 0.5 mM 3 isobutyl-1-methylxanthine (IBMX), 10 µg/ml insulin, 1 µM dexamethasone, and 10% FBS in DMEM for 14 days. The induction medium was changed every two days.
|
| Growth protocol |
Adipose-derived stem cells (ADSCs) were isolated from human flank lipoaspirate obtained in elective liposuction surgery procedures (Lonza, Switzerland) and maintained in Mesenchymal Stem Cell Growth Medium (Lonza).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from ADSCs using the RNeasy Mini kit (Qiagen Inc., Valencia, CA, USA). Quality and yield were verified by NimbleGen.
|
| Label |
Cy3
|
| Label protocol |
Labeling was performed by NimbleGen Systems Inc., Madison, WI USA, following their standard operating protocol. See www.nimblegen.com.
|
| |
|
| Hybridization protocol |
Hybridization was performed by NimbleGen Systems Inc., Madison, WI, USA following their standard operating protocol. See www.nimblegen.com.
|
| Scan protocol |
Scanning was performed by NimbleGen Systems Inc., Madison, WI USA, following their standard operating protocol. See www.nimblegen.com.
|
| Description |
ADSC-EGFP adipogenesis.
Image_Name: 220444A03
|
| Data processing |
The raw data (.pair file) was subjected to RMA (Robust Multi-Array Analysis; Irizarry et al. Biostatistics 4(2):249), quantile normalization (Bolstad et al. Bioinformatics 19(2):185), and background correction as implemented by NimbleScan software (Roche NimbleGen, Inc.). Students’ two-tail t-tests were conducted among the samples for each transcript and fold-change was determined. Transcripts whose abundance was significantly altered (P < 0.05) and an absolute fold change greater than 2 were defined as differentially regulated.
|
| |
|
| Submission date |
Jun 17, 2010 |
| Last update date |
Jun 21, 2010 |
| Contact name |
Masaki Mori |
| E-mail(s) |
mori9085@hotmail.com
|
| Phone |
81-6-6879-3901
|
| Fax |
81-6879-3909
|
| Organization name |
Osaka University
|
| Department |
Graduate School of Medicine
|
| Lab |
Division of Gene Therapy Science
|
| Street address |
2-2 Yamada-oka
|
| City |
SUita |
| State/province |
Osaka |
| ZIP/Postal code |
565-0871 |
| Country |
Japan |
| |
|
| Platform ID |
GPL8971 |
| Series (1) |
| GSE22429 |
Expression analysis of human adipose tissue stem cells (ADSCs) transduced with homeobox C8 (Hoxc8) or control vector, followed by treatment with adipogenic-induction media for 14 days |
|
