|
| Status |
Public on Dec 31, 2022 |
| Title |
Batch2_Paeoniflorin(High dose)_rep1 |
| Sample type |
SRA |
| |
|
| Source name |
A549
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: A549
dose: 10 uM
|
| Extracted molecule |
total RNA |
| Extraction protocol |
One mg of total RNA was processed for preparing mRNA sequencing library using MGIEasy RNA Directional Library Prep Kit (MGI Tech, China) according to manufacturer’s instruction.
Library was quantified by QauntiFluor ssDNA System (Promega, WI, USA).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
MGISEQ-2000RS |
| |
|
| Description |
Batch2_Paeoniflorin(High dose)_rep1
911
|
| Data processing |
Quality of raw sequence data was checked via the FastQC and adapter or low quality bases were trimmed with TrimGalore
After trimming, the reads were aligned to the human reference genome (hg38) using the STAR aligner
Read count and transcripts per million (TPM) were calculated as gene expression abundance using the RSEM software (v1.3.3) and gene annotation GRCh38.84 Gene Transfer Format (GTF) file
Genome_build: hg38
Supplementary_files_format_and_content: tab-delimited text files include count values for each Sample
|
| |
|
| Submission date |
Oct 20, 2021 |
| Last update date |
Dec 31, 2022 |
| Contact name |
Su-Jin Baek |
| Organization name |
KOREA INSTITUTE of ORIENTAL MEDICINE
|
| Street address |
1672, Yuseong-daero
|
| City |
Daejeon |
| ZIP/Postal code |
34054 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL30209 |
| Series (1) |
| GSE186218 |
Identification of Aurora B pathway involved in the anti-cancer effects by Paeoniae Radix extracts based on whole transcriptome analysis |
|
| Relations |
| BioSample |
SAMN22426029 |
| SRA |
SRX12702062 |
