|
| Status |
Public on Nov 11, 2022 |
| Title |
TGF_day_2 |
| Sample type |
RNA |
| |
|
| Source name |
MCF10A cells treated with TGFbeta for 2 days
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MCF10A
cell type: Normal breast epithelial cell line
treatment: TGFbeta for 2 days
|
| Treatment protocol |
10 ng/mL TGFbeta in complete growth medium for indicated time points
|
| Growth protocol |
Standard culture conditions for MCF10A
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen Rneasy kit
|
| Label |
biotin
|
| Label protocol |
standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
standard Affymetrix protocol
|
| Scan protocol |
standard Affymetrix protocol
|
| Description |
mRNA expression
|
| Data processing |
The arrays were normalized together using the Robust Multiarray Average (RMA) algorithm and a CDF (Chip Definition File) that maps the probes on the array to unique Entrez Gene identifiers
|
| |
|
| Submission date |
Jan 19, 2022 |
| Last update date |
Nov 13, 2022 |
| Contact name |
Andrew Emili |
| E-mail(s) |
aemili@bu.edu
|
| Organization name |
Boston University
|
| Department |
Biochemistry
|
| Lab |
CNSB
|
| Street address |
72 East Concord St
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02118 |
| Country |
USA |
| |
|
| Platform ID |
GPL17930 |
| Series (2) |
| GSE193985 |
Expression data from MCF10A cells treated with TGFβ for multiple time points [HuGene-2_0] |
| GSE194019 |
Expression data from MCF10A cells treated with TGFβ for multiple time points |
|
