|
| Status |
Public on Aug 31, 2010 |
| Title |
C2C12 growth condition, rep2 |
| Sample type |
RNA |
| |
|
| Source name |
C2C12 growth
|
| Organism |
Mus musculus |
| Characteristics |
media: growth
cell line: C2C12
|
| Treatment protocol |
To induce differentiation, cells were switched to DMEM 2% horse serum (Sigma) after 10 h ( 100% confluency).
|
| Growth protocol |
C2C12 cells were maintained at 20–70% confluency in Dulbecco’s Modified Eagles Medium (DMEM). supplemented with 10% fetal bovine serum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Standard Affymetrix protocol
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol
|
| Scan protocol |
Standard Affymetrix protocol
|
| Description |
Cells collected at 60–70% confluency
|
| Data processing |
We used the GC-bin method for background correction and applied quantile normalization. Probe set intensities and gene expression levels were derived from the probe logarithmic intensity error model (PLIER).
MoEx-1_0-st-v1.r2.pgf
MoEx-1_0-st-v1.r2.dt1.mm9.core.mps
|
| |
|
| Submission date |
Aug 30, 2010 |
| Last update date |
Aug 30, 2010 |
| Contact name |
Zhe Ji |
| E-mail(s) |
zhe.ji@northwestern.edu
|
| Organization name |
Northwestern University
|
| Street address |
303 E Superior St, Lurie 6-107
|
| City |
Chicago |
| State/province |
ILLINOIS |
| ZIP/Postal code |
60611 |
| Country |
USA |
| |
|
| Platform ID |
GPL6096 |
| Series (1) |
| GSE23871 |
Progressive lengthening of 3' untranslated regions of mRNAs by alternative polyadenylation during C2C12 differentiation |
|
