treatment: Allo spleen cells cell type: splenic CD11b+ myeloid cells
Growth protocol
Groups of wild type C57BL/6 mice were either untreated (naive, group D) or were injected i.p. with 20e6 allogeneic T cell depleted BALB/c splenocytes alone (group FN) with CTLA4-Ig (150µg i.p. once on day 2, Abatacept, Bristol-Myers Squibb, New York, NY, group DC), or CTLA4Ig plus recombinant EPO (5000 units/kg i.p. daily, epoetin alfa, Amgen, Thousand Oaks, CA, group DCE). On day 7, splenic CD11b+ myeloid cells were isolated by positive selection (kit #18970, STEMCELL, Vancouver, BC, Canada).
Extracted molecule
total RNA
Extraction protocol
RNA was isolated using TRIzol (Thermo Fisher Scientific, Waltham, MA) and cleaned using RNeasy Mini Kit (Qiagen, Hilden, Germany).
Label
N/A
Label protocol
N/A
Hybridization protocol
N/A
Scan protocol
N/A
Description
D2
Data processing
Gene expression profiling was then performed using the nCounter® Myeloid Innate Immunity Panel (NanoString Technologies, Seattle, WA). The normalized data were log2 transformed.
