Roots from (14+1)-day old A. thaliana ecotype Columbia (WT-2) seedlings
Biomaterial provider
Lehle Seeds, Round Rock, TX
Treatment protocol
24 hour exposure to hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) (>98% purity) added to liquid Murashige-Skoog culture medium at a rate of 150 mg/L from a 0.015% (v:v) DMSO stock solution
Growth protocol
Prior to dosing with RDX, 40 seeds in 500mL flasks containing 200 mL MS medium were grown 14 days at 25°C under a 16-hour photoperiod with constant shaking at 85 rpm in a growth chamber.
Extracted molecule
total RNA
Extraction protocol
Chang, S.; Puryear, J.; Cairney, J. Simple and efficient method for isolating RNA from pine trees. Plant Molecular Biology Reporter 1993, 11, 113-116. Plants were dipped in water to rinse, roots were dissected from hypocotyls, and flash-frozen in liquid nitrogen.
Description
Samples run in series in the same month and year as other samples in the GSE560 experimental series.
